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Using the clank-lz, an open-source PCB milling machine, to mill microfluidic chips that can be used to test different genetic circuits while maintaining bacteria alive. 

The system consists of three layers - an air control layer, a liquid culture layer, and a thin silicone membrane. The air and liquid layers are milled into 3mm polycarbonate sheets. The silicone membrane was cast using Ecoflex 00-30.

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©Laura Maria Gonzalez 2022

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